DE-MAN ROGOSA SHARPE BROTH (MRSB) A1 Quality | HX-MEDIA

DE-MAN ROGOSA SHARPE BROTH (MRSB) | HX-MEDIA

Product Overview

De-Man Rogosa Sharpe Broth (MRSB) is a highly enriched and selective liquid culture medium specifically formulated for the cultivation, enrichment, and maintenance of Lactobacillus species and other lactic acid bacteria (LAB). The medium was originally developed by De-Man Rogosa Sharpe Broth (MRSB) to provide optimal nutritional conditions for the growth of fastidious lactic acid-producing microorganisms commonly used in dairy, probiotic, food, and fermentation industries.

MRSB contains a balanced combination of peptones, beef extract, yeast extract, glucose, polysorbate 80, sodium acetate, ammonium citrate, magnesium sulfate, and manganese sulfate. These components collectively supply essential nutrients including amino acids, peptides, vitamins, minerals, fatty acids, and energy sources required for vigorous bacterial growth and metabolic activity.

MRSB is specially designed to support organisms that require rich nutritional supplementation and controlled growth conditions. Sodium acetate and ammonium citrate help suppress the growth of many unwanted bacteria, thereby improving selectivity for lactic acid bacteria. Polysorbate 80 enhances nutrient uptake and supports the metabolism of demanding microorganisms.

MRSB promotes excellent growth, acid production, and fermentation activity of:

• Lactobacillus species
• Lactococcus species
• Pediococcus species
• Leuconostoc species
• Other probiotic and fermentative bacteria

The medium is extensively used in:

• Dairy and probiotic industries
• Food microbiology laboratories
• Fermentation and biotechnology industries
• Pharmaceutical microbiology laboratories
• Research and academic institutions
• Quality control laboratories

MRSB is highly suitable for:

• Cultivation of probiotic organisms
• Enrichment of lactic acid bacteria
• Fermentation studies
• Starter culture preparation
• Microbial enumeration
• Quality control testing
• Preservation and maintenance of bacterial cultures
• Research involving beneficial microorganisms

The rich nutrient composition allows rapid bacterial multiplication and visible turbidity, making the broth highly effective for routine microbiological procedures and industrial fermentation applications.

Advantages of De-Man Rogosa Sharpe Broth

• Highly nutritious medium for lactic acid bacteria
• Excellent growth support for Lactobacillus species
• Promotes acid production and fermentation activity
• Selective formulation suppresses unwanted organisms
• Suitable for probiotic and dairy microbiology
• Reliable and reproducible performance
• Supports fastidious beneficial microorganisms
• Widely accepted in food and industrial microbiology laboratories

Due to its superior nutritional formulation, selective properties, and excellent growth-promoting capability, De-Man Rogosa Sharpe Broth (MRSB) is considered one of the most widely used and trusted media for the cultivation and study of lactic acid bacteria and probiotic microorganisms worldwide.

🔬 Key Features

• Selective medium for lactic acid bacteria
• Excellent growth promotion for Lactobacillus species
• Rich nutritional formulation
• Supports probiotic and fermentation studies
• Promotes acid production and metabolic activity
• Suitable for routine cultivation and maintenance
• Reliable and reproducible performance
• Widely used in food and dairy microbiology

🎯 Applications

• Cultivation of Lactobacillus species
• Probiotic microorganism research
• Dairy and fermented food microbiology
• Starter culture preparation
• Fermentation process studies
• Quality control testing
• Enumeration of lactic acid bacteria
• Biotechnology and industrial microbiology research

🧪 Composition (Typical)

Final pH: 6.5 ± 0.2

🛠️ How to Use

1. Suspend the required quantity of MRS Broth in distilled water.
2. Heat gently to dissolve completely.
3. Dispense into suitable tubes or flasks.
4. Sterilize by autoclaving at 121°C for 15 minutes.
5. Cool the medium before inoculation.
6. Inoculate the test organism aseptically.
7. Incubate at 30–37°C for 24–72 hours depending on the organism and application.

⏳Storage and Shelf Life

• Store in a cool, dry place away from direct sunlight
• Recommended storage temperature: 10–30°C
• Keep container tightly closed after use
• Protect from moisture and contamination
• Prepared medium should be stored under sterile conditions
• Use before the expiry date mentioned on the label

👍 Ideal For

✔ Dairy microbiology laboratories

✔ Probiotic manufacturers

✔ Food and beverage industries

✔ Fermentation industries

✔ Pharmaceutical microbiology laboratories