Czapek Dox Broth with Chloramphenicol A1 Quality – HX-Media

Czapek Dox Broth with Chloramphenicol – HX-Media

Product Overview

Czapek Dox Broth with Chloramphenicol is a selective, chemically defined liquid culture medium specifically formulated for the enrichment, cultivation, and isolation of fungi, particularly filamentous molds and certain yeasts, from samples containing mixed microbial populations.

This medium is based on the well-established Czapek Dox Broth formulation, which utilizes sucrose as the primary carbon source and sodium nitrate as the sole inorganic nitrogen source. This defined nutritional composition enables the selective growth of microorganisms capable of assimilating nitrate, making it especially suitable for fungi such as Aspergillus, Penicillium, and Fusarium species.

The addition of chloramphenicol, a broad-spectrum bacteriostatic antibiotic, enhances the medium’s selectivity by effectively inhibiting bacterial protein synthesis, thereby suppressing unwanted bacterial growth. This allows fungi to grow without competition, even when working with contaminated environmental, food, or pharmaceutical samples.

Being a liquid (broth) medium, it is particularly advantageous for bulk fungal cultivation, enrichment procedures, and biomass production, as well as for studies involving fungal metabolism, physiology, and nutrient utilization. It also serves as a preparatory enrichment step before subculturing onto solid media for isolation and identification.

Czapek Dox Broth with Chloramphenicol is widely used across environmental microbiology, food safety testing, pharmaceutical quality control, agricultural research, and industrial mycology laboratories due to its reproducibility, selectivity, and efficiency in promoting fungal growth while minimizing bacterial interference.

Its defined composition ensures consistent and reliable performance, Czapek Dox Broth making it an essential medium for laboratories requiring accurate fungal detection, isolation, and research outcomes.

🎯 Applications

• Isolation of fungi from contaminated samples (soil, air, water, food)
• Food spoilage and quality control testing
• Pharmaceutical microbial limit testing
• Agricultural and plant pathology studies
• Industrial microbiology (enzyme and metabolite production)
• Fungal biomass cultivation for research purposes
• Study of nitrate assimilation and fungal metabolism

🔬 Key Features

• Chemically defined medium for reproducible results
• Selective for fungi due to inorganic nitrogen utilization
• Chloramphenicol-enhanced selectivity against bacteria
• Czapek Dox Broth Supports a broad range of environmental and industrial fungi
• Suitable for enrichment and biomass production
• Enables metabolic and physiological studies
• Compatible with subsequent plating and identification techniques

🧪 Composition (Typical)

• Sucrose : 30.0
• Sodium Nitrate : 2.0
• Dipotassium Phosphate (K₂HPO₄) : 1.0
• Magnesium Sulfate (MgSO₄) : 0.5
• Potassium Chloride (KCl) : 0.5
• Ferrous Sulfate (FeSO₄) : 0.01
• Chloramphenicol : ~0.05 (or as required)
• Final pH : 7.3 ± 0.2 at 25°C

🛠️ How to Use

1. Weigh and suspend the required quantity of medium in distilled water
2. Czapek Dox Broth heat gently with agitation to dissolve completely
3. Sterilize by autoclaving at 121°C for 15 minutes
4. Cool the medium to 45–50°C
5. If chloramphenicol is not heat-stable in the formulation, add it aseptically after sterilization
6. Dispense into sterile culture tubes or flasks
7. Inoculate with the test sample under aseptic conditions
8. Incubate at 25–30°C for 3–7 days
9. Observe for turbidity, pellet formation, or mycelial mats
10. Subculture onto solid media (e.g., agar plates) if isolation is required

📦 Storage & Shelf Life

• Store dehydrated medium at 10–30°C in a dry environment
• Protect from moisture, heat, and direct light
• Keep container tightly sealed after use
• Prepared broth should be stored at 2–8°C and used within a short period
• Shelf life: Typically 2–3 years under recommended storage conditions

👍 Ideal For

✔ Food and beverage microbiology labs

✔ Environmental and industrial hygiene testing

✔ Isolation of fungi from heavily contaminated samples

✔ Routine fungal enrichment prior to plating