Buffered Sodium Chloride – Peptone solution pH 7.0 Harmonized – HX-MEDIA

Product Overview

Buffered Sodium Chloride Peptone Solution pH 7.0 (Harmonized) is a non-selective diluent and recovery medium used for the preparation, dilution, and resuscitation of microorganisms from food, water, pharmaceutical, and environmental samples. It is widely recommended in harmonized microbiological standards (ISO, FDA, pharmacopeial methods) for consistent and reproducible results.

The formulation contains sodium chloride to maintain osmotic balance and peptone as a nutrient source that helps in the recovery of stressed or injured microorganisms. The neutral pH (7.0 ± 0.2) ensures a stable environment that does not inhibit microbial viability during sample processing.

This medium is primarily used as a diluent in microbiological analysis, especially for serial dilution, sample homogenization, and total viable count procedures. It supports the survival of a wide range of microorganisms without promoting significant growth, ensuring accurate enumeration.

Buffered Sodium Chloride Peptone Solution is widely used in food safety testing, water analysis, pharmaceutical quality control, and research laboratories. It provides reliable recovery, stability, and compliance with international standards, making it an essential component in routine microbiological workflows.

🔬 Key Features

• pH-controlled at 7.0 ± 0.2

• Buffered to resist pH shifts during sample handling

• Sodium chloride provides osmotic balance

• Low-nutrient peptone stabilizes stressed microorganisms

• Clear, sterile, ready-to-use solution

🎯 Applications

• Diluent for food, environmental, cosmetic, and water microbiology

• Sample homogenization and preparation for ISO/EN methods

• Rinse/transport solution for swabs and surfaces

• Supports viable recovery for plate counts, MPN, and rapid tests

🧪 Composition (Typical)

• Sodium Chloride: ~8.5 g/L

• Peptone: ~1.0 g/L

• Buffer salts: ~3.5 g/L

• pH: 7.0 ± 0.2 at 25 °C

🛠️ How to Use

1. Aseptically dispense the solution into sterile containers or directly into the sample vessel.

2. Add sample at the required dilution ratio (commonly 1:10).

3. Mix or homogenize thoroughly to ensure even suspension.

4. Proceed with testing (plate counts, MPN, enrichment, or rapid methods) according to the applicable ISO/EN protocol.

⏳Storage and Shelf Life

• Store at 2–25 °C, protected from contamination

• Available as ready-to-use solution or dehydrated medium. Shelf life typically 12–36 months

👍 Ideal For

✔ ISO/EN-based microbiology workflows

✔ Reliable sample dilution and prep

✔ Stressed or sensitive microorganism handling

✔ Food, environmental, and cosmetic testing labs