Dichloran Rose Bengal Chloramphenicol Agar – Powerful Selective Agar for Reliable Fungal Testing – HX-MEDIA

Dichloran Rose Bengal Chloramphenicol Agar is a high-performance selective culture medium specially formulated for the isolation and enumeration of yeasts and molds. Commonly known as DRBC Agar, this medium is trusted by microbiology laboratories worldwide for its accuracy, selectivity, and reproducibility.

Designed to suppress bacterial contamination and control the spread of fast-growing fungi, Dichloran Rose Bengal Chloramphenicol Agar delivers clear, compact, and well-defined fungal colonies, making it ideal for routine testing and regulatory compliance.

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⭐ Why Choose Dichloran Rose Bengal Chloramphenicol Agar?

✔ Superior selectivity for yeasts and molds
✔ Prevents overgrowth of rapidly spreading fungi
✔ Inhibits bacterial interference
✔ Ensures accurate and easy colony counting
✔ Widely used in food, pharmaceutical & environmental testing

Whether you are performing routine quality control or detailed fungal analysis, Dichloran Rose Bengal Chloramphenicol Agar offers consistent and dependable results.

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🧪 Product Description (eCommerce Optimized)

Dichloran Rose Bengal Chloramphenicol Agar is formulated with carefully balanced selective agents that allow fungi to grow without competition. The inclusion of chloramphenicol effectively inhibits Gram-positive and Gram-negative bacteria, ensuring a clean background for fungal growth. Dichloran restricts the spreading of filamentous molds, while Rose Bengal dye slows growth and enhances visual contrast.

This combination results in small, discrete colonies that are easy to observe and enumerate, even in samples with heavy microbial load. The medium is especially effective for dry and semi-dry food products, where mold overgrowth is common.

Supplied in dehydrated powder form, Dichloran Rose Bengal Chloramphenicol Agar is easy to prepare and delivers a characteristic pink to rose-colored medium once poured, aiding rapid visual assessment.

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🧬 Principle of the Medium

The selective action of Dichloran Rose Bengal Chloramphenicol Agar is based on growth regulation and inhibition:

• Chloramphenicol suppresses bacterial growth

• Dichloran limits colony diameter of fast-growing molds

• Rose Bengal enhances differentiation and restricts excessive fungal spread

• Peptones and dextrose provide essential nutrients for fungal growth

This synergistic formulation ensures precise isolation and enumeration of fungi.

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🏭 Applications & Uses

🍞 Food Microbiology Testing

Ideal for detecting yeasts and molds in:

• Dairy products

• Bakery and confectionery items

• Cereals, grains, and flours

• Spices and dry foods

• Fruits, vegetables, and beverages

Helps prevent spoilage and maintain product quality.

💊 Pharmaceutical & Cosmetic Testing

Used for:

• Microbial limit testing

• Raw material analysis

• Finished product testing

• Clean room environmental monitoring

🌍 Environmental Monitoring

Suitable for:

• Air sampling

• Surface swabs

• Water testing

🎓 Research & Academic Laboratories

Used in:

• Fungal ecology studies

• Microbiology education

• Routine laboratory analysis

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📋 Technical Specification Sheet

Product Name : Dichloran Rose Bengal Chloramphenicol Agar

Abbreviation : DRBC Agar

Medium Type : Selective culture medium

Intended Use : Isolation and enumeration of yeasts and molds

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🧾 Typical Composition (per liter)

• Peptone – 5.0 g

• Dextrose – 10.0 g

• Potassium dihydrogen phosphate – 1.0 g

• Magnesium sulfate – 0.5 g

• Dichloran – 0.002 g

• Rose Bengal – 0.025 g

• Chloramphenicol – 0.1 g

• Agar – 15.0 g

(Exact composition may vary by manufacturer)

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🎨 Appearance

• Dehydrated medium: Pink to reddish powder

• Prepared medium: Pink to rose-colored gel

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⚖️ Final pH

5.6 ± 0.2 at 25°C

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🌡️ Incubation Conditions

• Temperature: 20–25°C

• Time: 3–5 days (up to 7 days if required)

• Atmosphere: Aerobic

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🔍 Interpretation of Results

• Yeasts: Small, smooth, cream to pink colonies

• Molds: Compact, well-defined colonies with restricted growth

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📦 Storage & Shelf Life

• Store dehydrated medium at 2–25°C

• Protect from moisture and light

• Prepared plates: Store at 2–8°C

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⚠️ Safety Information

• Contains chloramphenicol

• For laboratory use only

• Follow standard laboratory safety guidelines

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✅ Conclusion

Dichloran Rose Bengal Chloramphenicol Agar is an essential medium for laboratories requiring precision, selectivity, and compliance in fungal testing. Its proven formulation ensures reliable isolation and enumeration of yeasts and molds across food, pharmaceutical, cosmetic, and environmental applications.

For accurate fungal analysis and dependable results, Dichloran Rose Bengal Chloramphenicol Agar remains the gold-standard choice.