De-Man Rogosa Sharpe (MRS Broth) A1 Quality | HX-MEDIA

DE-MAN ROGOSA SHARPE BROTH (MRS Broth or MRSB) | HX-MEDIA

Product Overview

De-Man Rogosa Sharpe Broth (MRS Broth or MRSB) is a highly nutritious and selective microbiological culture medium specifically developed for the cultivation, enrichment, isolation, and maintenance of Lactobacillus species and other lactic acid bacteria (LAB). It is one of the most widely used media in food microbiology, dairy research, probiotic development, and fermentation studies due to its ability to provide optimal nutritional and environmental conditions for the growth of beneficial lactic acid-producing microorganisms.

MRS Broth contains a carefully balanced combination of peptones, beef extract, yeast extract, glucose, buffering salts, and essential minerals that collectively provide amino acids, vitamins, nitrogenous compounds, trace elements, and energy sources required for bacterial metabolism and growth. These nutrients support vigorous cell multiplication and help maintain the physiological activity of lactic acid bacteria during cultivation. The rich nutritional composition enables the recovery of stressed or low-level bacterial populations from diverse sample types, including dairy products, fermented foods, probiotic formulations, and environmental sources.

A key feature of MRS Broth is its selective formulation, which creates favorable growth conditions for Lactobacilli and related organisms while suppressing the growth of many undesirable bacteria. Components such as sodium acetate, ammonium citrate, and other selective agents contribute to the medium’s ability to promote lactic acid bacteria, making it highly effective for isolation and enrichment purposes. MRS Broth this selective nature allows researchers and quality control laboratories to obtain reliable and reproducible results when working with mixed microbial populations.

Key Nutritional Components

• Peptones provide essential amino acids and nitrogen sources.
• Yeast Extract and Beef Extract supply vitamins, growth factors, and minerals.
• Glucose serves as the primary energy source for bacterial growth.
• Buffering Salts help maintain stable pH conditions during fermentation.
• Magnesium and Manganese Salts support enzymatic activity and metabolism.
• Selective Agents favor the growth of lactic acid bacteria over competing microorganisms.

MRS Broth is extensively used in the dairy industry for the cultivation of starter cultures employed in the production of yogurt, cheese, fermented milk products, and other cultured foods. In probiotic manufacturing, it is used to propagate beneficial bacterial strains intended for dietary supplements and functional foods. The medium also plays a vital role in fermentation research, strain development, microbial physiology studies, and quality control testing.

MRS Broth ability to support robust bacterial growth makes it particularly useful for generating high cell densities required in industrial fermentation and probiotic production processes. It is also commonly used for maintaining stock cultures and studying the physiological and biochemical characteristics of lactic acid bacteria under controlled laboratory conditions.

Due to its nutrient-rich composition, selective properties, and proven reliability, MRS Broth remains the preferred medium for laboratories and industries involved in the cultivation and study of lactic acid bacteria. Its versatility, reproducibility, and effectiveness have established it as a standard medium for probiotic, dairy, food, and fermentation microbiology applications worldwide.

🔬 Key Features

• Specifically formulated for Lactobacillus and other lactic acid bacteria.
• Nutrient-rich composition for enhanced bacterial growth.
• Supports isolation, cultivation, and maintenance of LAB cultures.
• Widely used in probiotic and dairy microbiology.
• Suitable for fermentation and quality control studies.
• Reliable and reproducible results.

🎯 Applications

• Cultivation of Lactobacillus species.
• Isolation and enumeration of lactic acid bacteria.
• Probiotic research and development.
• Dairy product testing and starter culture preparation.
• Fermentation studies.
• Food microbiology and quality control.
• Academic and industrial microbiology research.

🧪 Composition (Typical)

• Peptones – Source of amino acids and nitrogen.
• Beef Extract & Yeast Extract – Provide vitamins and growth factors.
• Glucose – Primary energy source.
• Polysorbate 80 (Tween 80) – Enhances bacterial growth.
• Ammonium Citrate & Sodium Acetate – Selective growth-promoting agents.
• Magnesium Sulfate & Manganese Sulfate – Essential trace nutrients.
• Dipotassium Phosphate – Buffering agent.

🛠️ How to Use

1. Suspend the recommended quantity of medium in distilled water.
2. Heat MRS Broth gently with stirring until completely dissolved.
3. Dispense into suitable culture tubes or flasks.
4. Sterilize by autoclaving at 121°C for 15 minutes.
5. Cool before inoculation.
6. Inoculate the sample and incubate under appropriate conditions.

⏳Storage and Shelf Life

• Store at 10–30°C in a cool, dry place.
• Keep the container tightly closed after use.
• Protect from moisture and direct sunlight.
• Shelf Life: Up to 3 years when stored under recommended conditions.

👍 Ideal For

✔ Probiotic manufacturers.

✔ Fermentation industries.

✔ Pharmaceutical and biotechnology companies.

✔ Academic and research institutions.

✔ Microbial culture collection and maintenance facilities.