Tryptic Soya Broth (Soybean Casein Digest Broth) A1 Quality – HX-Media

Tryptic Soya Broth (Soybean Casein Digest Broth) – HX-Media

Product Overview

Tryptic Soya Broth (TSB), also referred to as Soybean Casein Digest Broth, is a highly nutritious, non-selective liquid culture medium formulated for the cultivation, enrichment, and maintenance of a broad spectrum of microorganisms, including aerobic, facultative anaerobic bacteria, and certain fungi and yeasts.

The medium is composed of enzymatic digests of casein (animal protein) and soybean meal (plant protein), which together provide a balanced and abundant supply of amino acids, peptides, nitrogen, vitamins, and trace nutrients essential for rapid microbial growth. This dual-source nutrient system enhances the growth of both fastidious and non-fastidious organisms, making Tryptic Soya Broth one of the most versatile and widely accepted general-purpose broths.

Tryptic Soya Broth is extensively used in pharmaceutical quality control, biotechnology, food and beverage testing, environmental monitoring, and clinical microbiology. Tryptic Soya Broth is officially recognized in major pharmacopoeias such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), JP (Japanese Pharmacopoeia), and IP (Indian Pharmacopoeia), particularly for sterility testing and microbial limit tests.

Due to its excellent buffering capacity, nutrient richness, and reproducibility, Tryptic Soya Broth supports luxuriant growth, making it ideal for inoculum preparation, culture propagation, and recovery of stressed or injured microorganisms.

🔬 Key Features

• Non-selective, general-purpose broth with high nutrient content
• Supports growth of bacteria, yeasts, and molds
• Promotes rapid and luxuriant microbial growth
• Suitable for fastidious organisms
• Recognized by USP, EP, JP, IP standards
• Excellent for sterility testing and microbial enumeration
• High batch-to-batch consistency and reproducibility
• Can be used as a base for preparing other media

🎯 Applications

🧪 Composition (Typical)

• Pancreatic Digest of Casein: 17.0 g

• Papaic Digest of Soybean Meal: 3.0 g

• Sodium Chloride: 5.0 g

• Dipotassium Phosphate: 2.5 g

• Glucose: 2.5 g

• Final pH (25°C): 7.3 ± 0.2

🛠️ How to Use

1. Weigh and suspend the required quantity of dehydrated medium in purified/distilled water.
2. Mix thoroughly and heat gently with agitation to dissolve completely.
3. Dispense into suitable containers (test tubes, flasks, or bottles).
4. Sterilize by autoclaving at 121°C for 15 minutes under 15 psi pressure.
5. Allow the medium to cool to room temperature.
6. Inoculate aseptically with the test organism or sample.
7. Incubate at 30–35°C or 35–37°C for 18–48 hours, depending on the organism.

⏳Storage and Shelf Life

👍 Ideal For

✔ Routine microbial cultivation.

✔ Sterility testing.

✔ Inoculum preparation.