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Price range: ₹905.00 through ₹8,050.00
Selenite F Broth works on the principle of selective inhibition and enrichment. Sodium selenite suppresses the growth of competing intestinal bacteria such as coliforms and Gram-positive organisms, while permitting the growth of Salmonella species.
Peptones provide nitrogenous nutrients, amino acids, and vitamins required for bacterial multiplication, whereas lactose helps maintain bacterial activity and reduces excessive toxicity of selenite during incubation.
During incubation, Salmonella organisms multiply rapidly and become enriched, improving their recovery when subcultured onto selective differential media such as XLD Agar, SS Agar, or Brilliant Green Agar.
100 g Bottle (makes ~1.5–2 liters of medium)
500 g Lab Pack
1 kg Bulk Pack
(Custom sizes available depending on supplier.)
Selenite F Broth (Twin Pack) is a highly selective enrichment medium extensively used for the isolation and recovery of Salmonella species from clinical, food, dairy, pharmaceutical, water, veterinary, and environmental samples. The medium is specifically formulated to enhance the growth of Salmonella organisms while suppressing the growth of competing intestinal flora and contaminating microorganisms commonly present in mixed specimens.
The medium contains sodium selenite as the primary selective agent, which inhibits many Gram-positive bacteria and several coliform organisms. This selective action allows Salmonella species to grow preferentially during incubation, thereby improving the chances of recovery even when the organisms are present in very small numbers.
Selenite F Broth is particularly valuable in samples where:
The nutrient-rich formulation contains peptones that provide amino acids, nitrogen compounds, vitamins, and essential growth nutrients necessary for bacterial multiplication. Lactose helps maintain metabolic activity and minimizes excessive toxicity caused by selenite during enrichment. Phosphate buffers help stabilize the pH of the medium and maintain favorable conditions for microbial growth.
The “Twin Pack” presentation generally offers improved convenience, stability, and ease of preparation by separating components when required. This helps maintain medium effectiveness and ensures better laboratory handling and storage efficiency.
Selenite F Broth is widely recommended for pre-enrichment and selective enrichment procedures before subculturing onto selective and differential agar media such as:
The medium is commonly used in:
Selenite F Broth plays a critical role in improving the recovery rate of Salmonella species from contaminated samples. During incubation, unwanted intestinal bacteria are inhibited while Salmonella organisms multiply rapidly, increasing their concentration and making subsequent isolation easier and more reliable.
The medium is especially useful for:
Because of its strong enrichment capability, Selenite F Broth is frequently used in routine microbiological quality control and public health monitoring programs worldwide.
Due to its dependable selectivity, enrichment efficiency, and broad laboratory applicability, Selenite F Broth (Twin Pack) remains one of the most commonly used enrichment media for the detection and isolation of Salmonella species in microbiology laboratories worldwide.
| Component | Quantity (g/L) |
|---|---|
| Peptone | 5.0 |
| Lactose | 4.0 |
| Sodium Phosphate | 10.0 |
| Sodium Biselenite / Sodium Selenite | 4.0 |
Final pH: 7.0 ± 0.2
✔ Clinical microbiology laboratories
✔ Food safety and quality control laboratories
✔ Pharmaceutical industries
✔ Water testing laboratories
✔ Veterinary microbiology laboratories
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Size | 100G, 1Kg, 500G |
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