Agar Agar (Plant Tissue Culture Grade) A1 Quality – HX-Media

Agar Agar (PTC Grade) – HX-Media

Product Overview

Agar Agar (Plant Tissue Culture Grade) is a highly purified, premium-quality gelling agent derived from selected species of marine red algae, specifically processed to meet the stringent requirements of agar agar plant tissue culture and biotechnology applications. It is widely recognized as a standard solidifying agent in plant culture media due to its excellent gel strength, clarity, stability, and inert nature.

This specialized grade of agar undergoes refined extraction and purification processes to remove unwanted impurities such as sulfates, heavy metals, pigments, and organic residues, which could otherwise inhibit plant cell growth or interfere with nutrient availability. As a result, it ensures a non-toxic and growth-supportive environment essential for sensitive plant tissues, explants, and cells cultivated under sterile conditions.

Agar Agar (Plant Tissue Culture Grade) forms a thermoreversible gel, meaning it melts at high temperatures (approximately 85–100°C) and solidifies upon cooling (around 32–40°C). This property makes it highly convenient for laboratory use, allowing easy preparation, sterilization, and dispensing of culture media without affecting its gelling capability. Once solidified, it provides a firm yet porous matrix that supports plant tissues physically while enabling the diffusion of water, nutrients, vitamins, and plant growth regulators throughout the medium.

Unlike other gelling agents, Agar Agar is chemically inert and not metabolized by most plant cells, ensuring that it does not alter the composition of the medium or compete with the plant tissue for nutrients. Its high transparency allows clear observation of root development, callus formation, contamination, and overall growth patterns, which is critical for research and quality control purposes.

This medium component is extensively used in combination with standard formulations such as Murashige and Skoog (MS), Gamborg B5, and other plant culture media, making it indispensable in micropropagation, callus induction, somatic embryogenesis, organogenesis, and plant regeneration studies. It is suitable for both research-scale and commercial-scale plant tissue culture operations, including nurseries and biotechnology industries.

Due to its batch-to-batch consistency, reproducibility, and compatibility with a wide range of plant species, Agar Agar (Plant Tissue Culture Grade) plays a critical role in ensuring uniform growth conditions and reliable experimental outcomes. Its performance directly influences the physical structure of the medium, water availability, and overall success of in vitro plant culture systems.

In summary, Agar Agar (Plant Tissue Culture Grade) serves as a fundamental and indispensable component in plant tissue culture, providing a stable, inert, and supportive gel matrix that enables controlled growth, differentiation, and development of plant tissues under laboratory conditions.

🔬 Key Features

• High purity, tissue culture grade quality
• Excellent gel strength and clarity
• Free from growth inhibitors and contaminants
• Provides stable and uniform gel structure
• Compatible with all plant tissue culture media
• Supports consistent and reproducible results

🎯 Applications

• Plant tissue culture and micropropagation
• Callus induction and organogenesis studies
• Somatic embryogenesis
• Seed germination and plant regeneration
• Research in plant biotechnology and genetic engineering
• Preparation of solid culture media

🧪 Composition (Typical)

• Purified agarose polysaccharides from red algae

• Free from salts, inhibitors, or other contaminants that affect plant tissue growth

• Forms a firm gel when dissolved in culture media at 0.6–1.0% (w/v)

🛠️ How to Use

1. Weigh required quantity (typically 6–10 g per liter of medium).
2. Add to distilled/deionized water or prepared culture medium.
3. Heat with continuous stirring until completely dissolved.
4. Adjust pH of the medium (if required).
5. Dispense into culture vessels.
6. Sterilize by autoclaving at 121°C for 15 minutes.
7. Allow to cool and solidify under sterile conditions.

⏳Storage and Shelf Life

• Store at 10–30°C in a dry, cool place
• Protect from moisture and direct sunlight
• Keep container tightly closed
• Shelf life: Typically 3–5 years under proper storage conditions

👍 Ideal For

✔ Plant tissue culture laboratories

✔ Micropropagation and plant biotechnology research

✔ Clonal propagation and germplasm preservation

✔ Callus culture, organogenesis, and somatic embryogenesis studies