Ashby’s Sucrose Broth – HX-MEDIA

Product overview

Ashby’s Sucrose Broth is a nitrogen-free liquid medium formulated for the enrichment and cultivation of free-living nitrogen-fixing bacteria (diazotrophs). By eliminating combined nitrogen sources and supplying sucrose broth as the primary carbon and energy source, the medium selectively supports microorganisms capable of fixing atmospheric nitrogen, while inhibiting non–nitrogen-fixing organisms.

This broth is widely used in soil microbiology, environmental studies, plant–microbe interaction research, and biofertilizer development. Its liquid format makes it ideal for enrichment, inoculum preparation, nitrogen-fixation studies, and metabolic assays involving diazotrophic bacteria.

🔬 Key Features

• Nitrogen-free formulation selectively supports growth of free-living nitrogen-fixing bacteria.

• Sucrose Broth as the sole carbon source encourages optimal growth of diazotrophs.

• Ideal for enrichment of nitrogen-fixing microorganisms from soil, water, and rhizosphere samples.

• Liquid format suitable for inoculum preparation, nitrogen-fixation studies, and metabolic assays.

• Consistent, defined composition ensures reproducible results in microbiology and agricultural research.

🧪 Composition (Typical)

• Sucrose – 20 g

• Dipotassium phosphate – 0.2 g

• Magnesium sulfate – 0.2 g

• Sodium chloride – 0.2 g

• Calcium carbonate – 5 g

• Distilled water – 1 L

• pH: 7.0 ± 0.2

🎯 Applications

• Enrichment of free-living nitrogen-fixing bacteria from soil, water, and plant-associated samples.

• Screening and studying diazotrophs involved in soil fertility and nutrient cycling.

• Preparation of inocula for further isolation or nitrogen-fixation experiments.

• Research in agricultural and environmental microbiology, especially related to biofertilizer development.

• Metabolic and physiological studies of nitrogen fixation in liquid culture conditions.

🛠️ How to Use

• Dissolve broth in water and adjust pH to 7.0 ± 0.2.

• Sterilize by autoclaving at 121°C for 15 min.

• Inoculate samples and incubate at 25–30°C for 3–7 days.

• Observe growth of nitrogen-fixing bacteria.

👍 Ideal For

✔ Microbiology Labs
✔ Schools & Universities
✔ Research Institutions
✔ Industrial QC Labs
✔ DIY Science Enthusiasts