Azospirillum Medium w/o Agar – HX-MEDIA

Product Overview

Azospirillum Medium (without agar) is a nitrogen-free liquid enrichment medium formulated for the selective isolation and cultivation of microaerophilic, nitrogen-fixing bacteria, particularly Azospirillum species from soil, rhizosphere, and plant root samples.

Azospirillum Medium is intentionally devoid of combined nitrogen, ensuring that only microorganisms capable of fixing atmospheric nitrogen can grow. It is enriched with a carbon source such as malate, along with essential mineral salts and trace elements that support metabolic activity and energy generation required for nitrogen fixation.

Under suitable incubation conditions, Azospirillum Medium exhibits a distinctive subsurface pellicle formation due to its microaerophilic nature, which serves as a key indicator for its presence and growth. The medium supports efficient enrichment even when the organism is present in low numbers.

This medium is extensively used in agricultural microbiology, soil fertility studies, environmental monitoring, plant-microbe interaction research, and biofertilizer industries. It is particularly valuable for isolating beneficial bacteria that contribute to nitrogen availability, plant growth promotion, and sustainable agriculture.

🔬 Key Features

• Optimized for liquid culture growth of Azospirillum Medium

• Supports nitrogen-fixing and plant-growth-promoting activity

• Promotes vigorous, motile bacterial growth

• Simple nutrient profile minimizes contamination from fast-growing bacteria

• Suitable for research, inoculum preparation, and fermentation studies

🎯 Applications

• Enrichment and cultivation of Azospirillum Medium species

• Studies on nitrogen fixation and plant-microbe interactions

• Production of biofertilizer inoculants

• Research on microbial ecology of rhizosphere

• Screening for plant-growth-promoting strains

🧪 Composition (Typical)

• Carbon source: Malate or glucose

• Nitrogen source: Ammonium salts or nitrates

• Mineral salts: Potassium, magnesium, and calcium salts

• Trace elements: Iron, manganese, and other micronutrients

• Vitamins and growth factors (as required)

• pH adjusted to ~6.5–7.0

🛠️ How to Use

1. Dissolve the recommended amount of dehydrated medium in distilled water.

2. Mix until fully dissolved.

3. Sterilize by autoclaving at 121 °C for 15 minutes.

4. Cool and inoculate with Azospirillum culture under sterile conditions.

5. Incubate at 28–30 °C with gentle shaking to maintain aeration.

⏳Storage and Shelf Life

• Dehydrated medium: Store at 2–8 °C, tightly sealed, away from moisture and light; shelf life 2–3 years.

• Prepared broth: Store at 2–8 °C; use within 2–4 weeks, discard if contamination or turbidity appears.

👍 Ideal For

✔ Microbiology and agricultural research laboratories

✔ Biofertilizer production and quality control

✔ Plant-microbe interaction studies

✔ Enrichment and maintenance of nitrogen-fixing Azospirillum cultures