Azotobacter Agar (Glucose) – HX-MEDIA

Product Overview

Azotobacter Agar (Glucose) is a selective, defined medium designed for the isolation, cultivation, and maintenance of free-living nitrogen-fixing bacteria, particularly Azotobacter species. It provides glucose as the primary carbon source, enabling these bacteria to grow robustly while utilizing atmospheric nitrogen in the absence of added fixed nitrogen.

This medium is widely used in soil microbiology, agricultural research, and microbial ecology to study nitrogen fixation and plant-growth-promoting bacteria.

🔬 Key Features

• Selective for Azotobacter and other nitrogen-fixing bacteria

• Supports growth without added nitrogen

• Contains essential salts and buffering agents for optimal bacterial growth

• Available as powder for easy preparation

• Suitable for research, agricultural studies, and teaching labs

🧪 Composition (Typical)

• Glucose (10–20 g/L): Primary carbon source for energy and growth of Azotobacter.

• Dipotassium Phosphate (0.2 g/L): Provides phosphate and potassium; acts as a buffering agent.

• Magnesium Sulfate (0.1 g/L): Source of magnesium and sulfur, essential for enzyme function.

• Sodium Chloride (0.05 g/L): Maintains osmotic balance.

• Potassium Sulfate (0.05 g/L): Supplies potassium and sulfur for growth.

• Calcium Carbonate (2 g/L): Provides calcium and helps maintain pH stability.

• Agar (15 g/L): Solidifying agent for plates.

• Distilled Water (1 L), pH ~7.0: Medium base, optimal for Azotobacter growth.

🎯 Applications

• Isolation of Azotobacter from soil and environmental samples

• Research on nitrogen-fixing bacteria and plant growth-promoting microorganisms

• Maintenance of pure cultures of free-living nitrogen-fixing bacteria

• Educational and laboratory training in microbiology

🛠️ How to Use

• Dissolve the recommended quantity of medium (~40–45 g/L) in distilled water.

• Heat to dissolve completely and autoclave at 121°C for 15 minutes.

• Pour into sterile Petri plates and allow to solidify.

• Inoculate samples for isolation, enumeration, or research studies.

👍 Ideal For

✔ Microbiology Labs
✔ Schools & Universities
✔ Research Institutions
✔ Industrial QC Labs
✔ DIY Science Enthusiasts