Azotobacter Broth (Glucose) – HX-MEDIA

Product Overview

Azotobacter Broth (Glucose) is a nitrogen-free enrichment medium designed for the cultivation and proliferation of free-living nitrogen-fixing bacteria, particularly Azotobacter Broth. The absence of combined nitrogen sources encourages the growth of organisms capable of fixing atmospheric nitrogen.

Glucose acts as the primary carbon and energy source, promoting active growth and metabolic activity. The broth medium allows for rapid multiplication and enrichment of Azotobacter Broth species, making it suitable for further isolation and study.

This medium is widely used in soil microbiology, agricultural research, environmental studies, and biofertilizer development laboratories. It provides efficient growth conditions, reliable enrichment, and consistent performance, making it ideal for routine and research applications involving nitrogen-fixing bacteria.

🎯 Applications

• Isolation and cultivation of Azotobacter spp. from soil, water, and rhizosphere samples

• Enrichment of nitrogen-fixing bacteria for microbiological and agricultural research

• Qualitative and quantitative estimation of nitrogen fixation potential

• Teaching and demonstration in environmental microbiology, soil science, and biotechnology labs

• Starter medium for downstream assays (e.g., pigment production, poly-β-hydroxybutyrate formation)

🔬 Key Features

• Nitrogen-free formula supporting selective growth of aerobic nitrogen fixers

• Glucose-based energy source for enhanced biomass yield

• Clear, homogenous broth facilitating optical density measurements

• Suitable for environmental, agricultural, and academic laboratories

🧪 Composition (Typical)

• Glucose – 20.0 g

• Dipotassium phosphate – 0.2 g

• Magnesium sulfate – 0.2 g

• Sodium chloride – 0.2 g

• Potassium sulfate – 0.1 g

• Ferrous sulfate – 0.006 g

• Calcium carbonate – 5.0 g

• Distilled water – 1 L

🛠️ How to Use

1. Add your environmental sample (commonly soil or root-zone material) to the nitrogen-free broth.

2. Because the broth lacks combined nitrogen, only nitrogen-fixing bacteria like Azotobacter Broth will grow well.

3. Look for growth such as a surface pellicle or turbidity, indicating active nitrogen fixers.

4. The enriched culture can then be observed, studied, or transferred to other media for further work.

Note: For exact laboratory procedures, follow your facility’s official protocols and the manufacturer’s instructions.

⏳Storage and Shelf Life

• Store dehydrated medium at 2–30°C, tightly sealed

• Keep away from moisture, heat, and light

• Long shelf life under proper storage (commonly 2–3 years)

• Store prepared broth at 2–8°C and use promptly

👍 Ideal For

✔ For laboratory use only

✔ Handle with standard microbiological safety procedures

✔ Not for human or animal consumption