Plate Count Agar [Tryptone Glucose Yeast Extract Agar] – HX-MEDIA

Product Overview

Plate Count Agar (PCA), also known as Tryptone Glucose Yeast Extract Agar, is a general-purpose, non-selective culture medium used for the enumeration of viable microorganisms in food, water, dairy, and environmental samples. It supports the growth of a wide range of heterotrophic bacteria, making it ideal for determining the total microbial load.

The medium contains tryptone as a source of amino acids, glucose as an ऊर्जा source, and yeast extract providing vitamins and growth factors. Its balanced formulation promotes consistent colony development, allowing accurate counting of microbial populations.

Plate Count Agar is widely used in food safety testing, dairy industries, water analysis, pharmaceutical quality control, and research laboratories, especially for standard plate count (SPC) or aerobic plate count (APC) methods.

🔬 Key Features

• Standard medium for total viable counts

• Broad bacterial recovery

• Clear colony development

• ISO/APHA recommended

🎯 Applications

• Total aerobic bacterial count

• Food, dairy, water & pharma QC

• Environmental and industrial monitoring

🧪 Composition (Typical)

• Tryptone: 5.0 g

• Yeast Extract: 2.5 g

• Glucose: 1.0 g

• Agar: 15.0 g

• Final pH: ~7.0 ± 0.2 at 25°C

🛠️ How to Use

1. Dissolve medium, sterilize at 121°C for 15 min.

2. Cool, pour plates, inoculate sample.

3. Incubate 30–37°C for 24–48 hrs and count colonies.

⏳Storage and Shelf Life

• Store powder at 2–8°C, dry and protected from light.

• Use freshly prepared plates; store at 2–8°C and use within a few days.

👍 Ideal For

✔ Routine microbial load testing

✔ Food, water, and beverage QC

✔ Dairy and pharma laboratories

✔ Environmental and industrial monitoring