Acetobacter Mannitol Agar – HX-MEDIA

Product Overview

Acetobacter Mannitol Agar is a selective solid culture medium designed for the isolation and cultivation of acetic acid bacteria, particularly Acetobacter. The medium contains mannitol as the primary carbon source, supporting the growth and metabolic activity of target organisms.

It promotes the formation of characteristic colonies of Acetobacter species, which are known for their role in oxidizing alcohols into acetic acid, a key process in vinegar production and fermentation industries. Selective agents may be incorporated to inhibit unwanted microbial flora, enhancing isolation efficiency.

This medium is widely used in food and beverage industries, fermentation processes, quality control laboratories, and microbiological research. Acetobacter Mannitol Agar provides reliable growth, clear colony morphology, and consistent results, making it suitable for routine isolation and study of acetic acid bacteria.

🔬 Key Features

• Selective for acid-tolerant Acetobacter

• Mannitol-based carbon source promotes acetic acid production

• Solid agar allows easy observation of colony morphology

• Supports routine microbiological and fermentation research

🎯 Applications

• Isolation and maintenance of Acetobacter spp. from vinegar, kombucha, or fermented products

• Quality control in fermentation industries

• Study of acetic acid bacteria in food, beverage, and industrial microbiology

• Detection of acid-producing aerobic bacteria in environmental or industrial samples

🧪 Composition (Typical)

• Mannitol – 20 g

• Peptone – 5 g

• Yeast extract – 10 g

• Calcium carbonate – 20 g

• Agar – 15–20 g

• Distilled water – 1 L

🛠️ How to Use

• Spread or streak your sample onto the agar.

• Incubate aerobically at 28–30°C.

• Observe small, glossy, acid-producing Acetobacter colonies.

• Count, examine, or transfer colonies as needed.

⏳Storage and Shelf Life

• Store dehydrated medium at 2–30°C in a dry, sealed container

• Protect from moisture, heat, and light

• Prepared plates should be stored at 2–8°C and used promptly

👍 Ideal For

✔ For laboratory and industrial use only
✔ Handle using standard microbiological safety practices
✔ Not intended for human or animal consumption