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Price range: ₹1,040.00 through ₹79,050.00
Asparagine Broth is a defined, simple nitrogen-rich liquid medium primarily used for the selective enrichment and detection of Pseudomonas species, especially Pseudomonas aeruginosa. It is valued for its minimal composition, which allows organisms capable of utilizing asparagine as a sole nitrogen source and glycerol (or other carbon sources) as the sole carbon source to grow, while inhibiting many other bacteria.
The broth contains minimal ingredients, with asparagine as the defining component:
Asparagine provides nitrogen that only certain bacteria can utilize.
Glycerol or glucose (depending on formulation) provides carbon.
This minimal environment suppresses growth of non–Pseudomonads.
Fluorescent pigments produced by Pseudomonas species serve as indicators of growth.
100 g Bottle (makes ~1.5–2 liters of medium)
500 g Lab Pack
1 kg Bulk Pack
(Custom sizes available depending on supplier.)
Asparagine Broth is a defined, selective liquid culture medium designed for the enrichment and detection of Pseudomonas species, particularly Pseudomonas aeruginosa. Its minimal composition—featuring L-asparagine as the sole nitrogen source and glycerol or glucose as the primary carbon source—allows microorganisms with specialized metabolic capabilities to grow while inhibiting most competing flora.
This medium is widely used in clinical, industrial, environmental, and research laboratories for screening water, soil, pharmaceutical products, and clinical samples for Pseudomonas contamination.
Selective enrichment for Pseudomonas spp., especially P. aeruginosa.
Defined minimal formula using L-asparagine as the sole nitrogen source.
Supports pigment production (pyocyanin, pyoverdine) for easy presumptive ID.
High specificity, limiting growth of non-Pseudomonas organisms.
Suitable for environmental and industrial testing (water, pharma, cosmetics).
Easy preparation and consistent performance.
Enrichment and detection of Pseudomonas aeruginosa in clinical, environmental, and industrial samples.
Microbiological quality control in water, pharmaceutical, cosmetic, and food testing.
Research studies on nitrogen metabolism and selective growth of Pseudomonas species.
Preliminary identification via pigment production (pyocyanin and fluorescent pyoverdine).
Environmental monitoring of soil, wastewater, and other natural sources for Pseudomonas contamination.
L-Asparagine – 5 g
Dipotassium phosphate – 1.5 g
Magnesium sulfate – 0.2 g
Glycerol or glucose – 10–20 mL
Distilled water – 1 L
pH: 7.0 ± 0.2
Dissolve the measured components in distilled water.
Adjust the pH to ~7.0.
Dispense into suitable tubes or flasks.
Sterilize by autoclaving at 121°C for 15 minutes.
Dehydrated medium: Store tightly sealed at 15–30°C in a dry environment
Prepared medium: Store at 2–8°C; avoid freezing
Shelf life: Typically 2–3 years (dehydrated), depending on manufacturer
âś” Microbiology Labs
âś” Schools & Universities
âś” Research Institutions
âś” Industrial QC Labs
âś” DIY Science Enthusiasts
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Size | 100G, 10KG, 1Kg, 500G, 5KG |
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