Luria Bertani Broth A1 Quality – HX-Media

Luria Bertani Broth A1 Quality – HX-Media

Product Overview

Luria Bertani Broth is a high-performance, laboratory-grade microbiological culture medium developed to support the growth, cultivation, isolation, and maintenance of microorganisms under controlled conditions. It is formulated using a precisely balanced combination of carbon sources (such as carbohydrates), nitrogenous compounds (peptones or protein digests), essential vitamins, inorganic salts, and growth factors, all of which are critical for cellular metabolism, enzyme activity, and microbial proliferation.

The carefully optimized composition ensures that microorganisms receive readily available nutrients required for rapid growth, efficient energy production, and biosynthesis of cellular components. Carbon sources act as the primary energy supply, while nitrogen sources provide amino acids and peptides necessary for protein synthesis. Mineral salts help maintain osmotic balance, pH stability, and enzymatic functions, creating a favorable and stable growth environment.

Depending on the formulation, the medium can function as a general-purpose, selective, enrichment, or differential medium, enabling laboratories to target specific groups of microorganisms or support a broad microbial population. This versatility makes it highly suitable for isolation, enumeration, identification, and maintenance of microbial cultures across diverse applications.

Luria Bertani Broth is typically supplied in a dehydrated powder form, which ensures extended shelf life, ease of transportation, and convenient storage. Upon reconstitution with distilled water and sterilization, it forms a homogeneous and reproducible culture system, ensuring batch-to-batch consistency and reliable results. Its formulation is designed to withstand standard sterilization conditions without significant degradation, maintaining its nutritional integrity and performance.

Luria Bertani Broth is extensively used in clinical diagnostics, pharmaceutical quality control, food and beverage microbiology, water testing, environmental monitoring, and academic and industrial research laboratories. It plays a vital role in detecting microbial contamination, studying microbial physiology and metabolism, conducting sterility and limit tests, and supporting fermentation and biotechnological processes.

Additionally, Luria Bertani Broth offers flexibility for customization, allowing supplementation with antibiotics, indicators, or specific nutrients depending on the intended application. Its consistent quality, adaptability, and proven reliability make it an essential component in modern microbiology laboratories.

Luria Bertani Broth overall, this culture medium provides a robust, versatile, and scientifically validated platform for achieving accurate, reproducible, and efficient microbial growth and analysis, meeting the demands of both routine testing and advanced research applications.

🔬 Key Features

• Supports rapid growth of non-fastidious bacteria
• Ideal for E. coli cultivation and cloning work
• Promotes high cell density and biomass yield
• Simple, reliable, and widely standardized
• Suitable for genetic and molecular biology applications
• Can be supplemented with antibiotics or inducers

🎯 Applications

• Supports rapid growth of non-fastidious bacteria
• Ideal for E. coli cultivation and cloning work
• Promotes high cell density and biomass yield
• Simple, reliable, and widely standardized
• Suitable for genetic and molecular biology applications
• Can be supplemented with antibiotics or inducers

🧪 Composition (Typical)

• Tryptone: 10 g

• Yeast extract: 5 g

• Sodium chloride: 10 g

• pH: 7.0 ± 0.2

🛠️ How to Use

1. Dissolve 25 g of medium in 1 liter distilled water
2. Luria Bertani Broth mix thoroughly to ensure complete dissolution
3. Dispense into flasks or culture tubes
4. Sterilize by autoclaving at 121°C for 15 minutes
5. Cool to room temperature
6. Add antibiotics if required (after cooling)
7. Inoculate aseptically with bacterial culture
8. Incubate at 35–37°C (shaking recommended for aeration)

⏳Storage & Shelf Life

• Store in a cool, dry place (10–30°C)
• Keep tightly closed, away from moisture
• Store in a cool, dry place (10–30°C)
• Keep tightly closed, away from moisture
• Dehydrated: 2–3 years
• Prepared: 1–2 weeks

👍 Ideal For

• Molecular biology labs

• Recombinant protein and plasmid workflows