Sabouraud Dextrose Broth with Chloramphenicol A1 Quality – HX-Media

Sabouraud Dextrose Broth with Chloramphenicol – HX-Media

Product Overview

Sabouraud Dextrose Broth with Chloramphenicol is a selective liquid culture medium formulated for the cultivation and enrichment of yeasts and molds while inhibiting bacterial contamination.

This medium is based on Sabouraud Dextrose formulation, which contains a high concentration of dextrose and an acidic pH that favors fungal growth. The addition of chloramphenicol, a broad-spectrum antibiotic, enhances selectivity by suppressing the growth of most Gram-positive and Gram-negative bacteria.

It is widely used in clinical, pharmaceutical, food, and environmental microbiology laboratories for the isolation, cultivation, and maintenance of fungal organisms, including dermatophytes, Candida species, and other pathogenic fungi.

🔬 Key Features

• Selective medium for yeasts and molds

• Contains chloramphenicol to inhibit bacterial growth

• High dextrose concentration promotes fungal growth

• Acidic pH enhances selectivity

• Suitable for clinical and industrial applications

• Reliable and reproducible results

🎯 Applications

• Isolation and cultivation of fungi (yeasts and molds)

• Detection of fungal contamination in food and pharmaceuticals

• Clinical diagnosis of fungal infections

• Environmental monitoring

• Maintenance and enrichment of fungal cultures

🧪 Composition (Typical)

• Dextrose: ~40.0 g

• Peptone: ~10.0 g

• Chloramphenicol: ~0.05 g (50 mg)

Final pH: ~5.6 ± 0.2 at 25°C

🛠️ How to Use

1. Suspend the required amount of medium in distilled water (as per label instructions).

2. Heat gently with stirring to dissolve completely.

3. Dispense into suitable containers.

4. Sterilize by autoclaving at 121°C for 15 minutes.

5. Cool to about 45–50°C (if antibiotic is added separately, add aseptically at this stage).

6. Inoculate the sample under aseptic conditions.

7. Incubate at 25–30°C for 2–5 days or longer depending on organism growth.

⏳Storage and Shelf Life

• Store dehydrated medium at 10–30°C in a dry place

• Protect from light and moisture (antibiotic is light-sensitive)

• Keep container tightly closed

• Prepared medium should be used fresh or stored at 2–8°C for short-term use

• Shelf life: 2–3 years under recommended storage conditions

👍 Ideal For

✔ Food, cosmetic, and pharmaceutical QC labs

✔ Environmental and surface fungal testing

✔ Clinical and research labs working with yeasts and molds